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Objective:To observe the application value of duodenal suspension traction technology in laparoscopic duodenal anastomosis.Methods:80 cases of congenital intestinal atresia and stenosis A children treated in our hospital from May 2013 to May 2015were selected by retrospective study law,and were divided into the control group (not used) group and the observation group (with group) according to their surgery whether duodenum suspension traction technology,40 cases in each group.All patients were laparoscopic surgical approach for the three holes.The general surgery situation the incidence of postoperative complications and associated laboratory parameters in the two groups were compared.Results:The operative time,the initial feeding time,the time return to normal diet and hospital stay in observation group were shorter than those in the control group,and the number of blood loss and cases converted to open surgery in the procedure was as well as was significantly less than the control group (P＜0.05);Before surgery,the level of WBC,NEU％,and inflammatory cells factors as PCT,IL-6CPR in the two groups had no significant difference;five days after surgery,the level of WBC,NEU％ and IL-6 in the two groups were all decreased,but the observation group decreased more significantly(P＜0.05);observation group The rate of anastomotic leakage,anastomotic dehiscence,the incidence of adhesions,wound infection and other complications in observation group were significantly lower than the control group,the difference was statistically significance(P＜0.05).Conclusion:The duodenum suspension traction technology in laparoscopic duodenal anastomosis,can reduce accidental injury,then reduce the operation rate,thus shortening the duration of surgery in children,the time required for the first time to eat and hospitalization time for the expansion of the surgical field,and which significantly can reduce the incidence of postoperative infection and other complications in children.
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Objective To explore the problems and countermeasures during the implementation of after-sale service of the medical equipment.Methods The common problems and their causes during after-sale service were analyzed,and some countermeasures were proposed from the aspects of signing purchase contract,acceptance check,operation quality,overall evaluation,personnel and etc.Results Strengthened after-sale service contributed to enhancing the medical equipment in operation quality,medical safety and efficiency.Conclusion It is important to take corresponding measures during after-sale service to improve operation quality and decrease operation cost of the medical equipment.
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Objective To detect the mutations of RET proto-oncogene in patients with medullary thyroid carcinoma (MTC). Methods Twelve patients with MTC confirmed by pathology and two clinically suspected patients were recruited. Total genomic DNA was extracted from peripheral blood for PCR. PCR products of exon 10, exon 11 and exon 16 of the RET proto-oncogene were purified and direct gene sequencing was performed. Results The genomic mutations were detected in 8 patients, in which direct gene sequencing analysis detected a mutation of TGC to CGC at codon 618 in exon 10 in 1 patient, a mutation of TGC to TAC at codon 634 in exon 11 in 2 cases, a mutation of TGC to CGC at codon 634 in exon 11 in 3 patients and a mutation of ATG to ACG at codon 918 in exon 16 in the remaiming 2 cases. Conclusion MTC can be diagnosed at gene level by direct gene sequencing analysis. It is possible to diagnose MTC before operation by means of molecular genetic analysis.
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ObjectiveTo investigate the relationships between insulin-like growth factor-Ⅰ (IGF-Ⅰ) and osteoprotegerin (OPG), RANKL, and bone mineral density (BMD) in healthy women. MethodsBMD of lumbar spine and femoral neck were measured in 504 healthy women by dual energy X-ray absorptiometry and their serum levels of IGF-Ⅰ, OPG, RANKL were also determined. ResultsAge was negatively correlated with serum level of IGF-Ⅰ in healthy women (r=-0.702, P
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Objective To search the single nucleotide polymorphism (SNP) in exons of osteoprotegerin gene, and to analyse the relationship between SNP and bone mineral densities (BMD) in postmenopausal women. Methods Using PCR and direct sequencing to identify SNP and genotypes in 205 postmenopausal women. BMD at lumbar spine (L 2 4 ) and femoral neck (FN) were measured by dual energy X ray absorptiometry. Serum osteocalcin (BGP), osteoprotegerin (OPG), osteoprotegerin ligand (RANKL) and urinary N telopeptides of type Ⅰ collagen (NTx) were also measured. Results One SNP, G1181C, was found in exon 1 of OPG gene. The frequencies of G1181C genotypes in 205 postmenopausal women were 0.566, 0.346, and 0.088 for the genotypes GG, GC and CC respectively. BMD at lumbar spine (L 2 4 ) of CC genotype was significantly higher than GC and GG genotypes (P